Protocorm like Bodies and Plantlet Regeneration from Demdrobium formosum Leaf Callus
نویسندگان
چکیده
منابع مشابه
Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids.
Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by wounding of pr...
متن کاملProtocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite.
The distinct reproductive program of orchids provides a unique evolutionary model with pollination-triggered ovule development and megasporogenesis, a modified embryogenesis program resulting in seeds with immature embryos, and mycorrhiza-induced seed germination. However, the molecular mechanisms that have evolved to establish these unparalleled developmental programs are largely unclear. Here...
متن کاملCallus Mediated Organogenesis and Plant Regeneration of Cinnamomum Tamala Nees. (lauraceae) from Leaf and Zygotic Embryos
Cinnamomum tamala is a multipurpose economically important plant. The leaves and bark are used as spice and contributes to the different ‘Ayurvedic’ drug preparation. Due to over-exploitation for spice and medicines, the species is vulnerable in its natural habitat. In the present study, a successful attempt was made for callus mediated plant regeneration of the species from leaf and zygotic em...
متن کاملAmmonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium
Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...
متن کاملCallus induction and plant regeneration from leaf explants of jojoba [Simmondsia chinensis (Link) Schneider]
Sunil Kumar 1,2 , Manisha Mangal 3 , A K Dhawan 4 and Narender Singh 2 * Centre for Plant Biotechnology, CCS Haryana Agricultural University New Campus, Hisar 125 004, India Department of Botany, Kurukshetra University, Kurukshetra 136 119, India Central Institute of Post-Harvest Engineering and Technology, Ludhiana 141 001, India CCS Haryana Agricultural University, Regional Research Station, ...
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ژورنال
عنوان ژورنال: Asian Journal of Plant Sciences
سال: 2003
ISSN: 1682-3974
DOI: 10.3923/ajps.2003.955.957